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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
22/09/2016 |
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Data da última atualização: |
06/10/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, M. R. A. dos; SOUZA, C. A. de. |
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Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; Carolina Augusto de Souza. |
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Título: |
Dedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Australian Journal of Basic and Applied Sciences, v. 10, n. 12, p. 362-368, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Background:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 μ M 2,4-D + 0.44 μ MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used. MenosBackground:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuum... Mostrar Tudo |
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Palavras-Chave: |
Callogenesis; Calogêneses; Growth curve; Metabolismo secundário. |
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Thesagro: |
Curva de Crescimento; Pimenta. |
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Thesaurus Nal: |
secondary metabolites. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147697/1/Differentiation-SANTOS-2016.pdf
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Marc: |
LEADER 02419naa a2200217 a 4500
001 2053310
005 2016-10-06
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. R. A. dos
245 $aDedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna.$h[electronic resource]
260 $c2016
520 $aBackground:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 μ M 2,4-D + 0.44 μ MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used.
650 $asecondary metabolites
650 $aCurva de Crescimento
650 $aPimenta
653 $aCallogenesis
653 $aCalogêneses
653 $aGrowth curve
653 $aMetabolismo secundário
700 1 $aSOUZA, C. A. de
773 $tAustralian Journal of Basic and Applied Sciences$gv. 10, n. 12, p. 362-368, 2016.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 48 | |
| 5. |  | SOUZA, C. A. de; ROCHA, R. B.; MORAES, M. S.; SPINELLI, V. M.; ALVES, E. A. Caracterização da peneira média e percentual de grão tipo moca DE Coffea Canephora das variedades botânicas conilon e robusta. Enciclopédia Biosfera, Centro Científico Conhecer, Goiânia, v. 14 n. 26, p. 156-166, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Rondônia. |
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| 10. | | SANTANA, P. G.; SPINELLI, V. M.; ANDRADE, T. R.; SOUZA, C. A. de; MORAES, M. S.; ROCHA, R. B. Avaliação da variabilidade de características foliares entre genótipos de Coffea canephora. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 11.; ENCONTRO DE PÓS-GRADUAÇÃO, 6., 2021, Porto Velho. Anais... Porto Velho: Embrapa Rondônia, 2021. p. 57-62. Editores: Ana Karina Dias Salman; Henrique Nery Cipriani.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 11. | | SOUZA, C. A. de; TEIXEIRA, A. L.; TORRES, J. D.; SILVA, C. A.; ESPINDULA, M. C.; ROCHA, R. B. Adaptability and stability of Coffea arabica lines in the western Amazon. Coffee Science, v. 14, n. 2, p. 240-249, apr./jun. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Rondônia. |
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| 12. | | GREGO, C. R.; CRISCUOLO, C.; SOUZA, C. A. DE; BRITO, M. H. S.; PRETTO, S. L.; MOTOKI, S. H. O café na Região Metropolitana de Campinas. In: CRISCUOLO, C. (Ed.). Atlas escolar da Região Metropolitana de Campinas. 2. ed. Brasília, DF: Embrapa, 2018. v. 2, cap. IV, p. 96-121.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Territorial. |
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| 13. | | SANTOS, M. R. A. dos; SOUZA, C. A. de; ROCHA, J. F. da; ARAUJO, L. V. de; ESPINDULA, M. C. Comparison of Economic Efficiency between in Vitro and Field Methods for Vegetative Propagation of Coffea Canephora. Australian Journal of Basic and Applied Sciences, Pakistan, v. 9, n. 20, p. 1 - 7, jun. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Rondônia. |
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| 14. | | MORAIS, J. A. de; ROCHA, R. B.; ALVES, E. A.; ESPINDULA, M. C.; TEIXEIRA, A. L.; SOUZA, C. A. de. Beverage quality of Coffea canephora genotypes in the western Amazon, Brazil. Acta Scientiarum. Agronomy, v. 43, e52095, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Rondônia. |
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| 18. | | SOUZA, C. A. de; MAGALHÃES, G. M. de O.; SOUZA, P.; PAZ, E. S.; SANTOS, M. R. A. dos. Padrão de crescimento de calos friáveis de folhas, entrenó e nó de Capsicum annuum var. annuum cv. iberaba Jalapeño. In: JORNADA CIENTÍFICA CEDSA, 10., 2015, Porto Velho. [Anais...]. Porto Velho: Unir, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 20. | | MORAES, M. S.; SANTANA, P. G.; SOUZA, C. A. de; DEPOLO, R. P.; ESPINDULA, M. C.; ROCHA, R. B. Desempenho das cultivares Robustas Amazônicos no município de Alta Floresta do Oeste, Rondônia. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 11.; ENCONTRO DE PÓS-GRADUAÇÃO, 6., 2021, Porto Velho. Anais... Porto Velho: Embrapa Rondônia, 2021. p. 21. Editores: Ana Karina Dias Salman; Henrique Nery Cipriani.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 48 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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